| 质粒名称: | MSGV Hu Acceptor |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64269 |
| 存储实验室: | David Ott |
|
载体骨架基本信息 |
|
| 载体名称: | pMSGV |
| 载体出品公司: | Richard Morgan NCI |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | TCR克隆插入物：tcrv区基因受体/恒定区基因 |
| 载体类型: | 逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | MSGV Hu Acceptor was a gift from David Ott (Addgene plasmid # 64269 ; http://n2t.net/addgene:64269 ; RRID:Addgene_64269) |
| 参考文献部分: | Production of retroviral constructs for effective transfer and expression of T-cell receptor genes using Golden Gate cloning. Coren LV, Jain S, Trivett MT, Ohlen C, Ott DE. Biotechniques. 2015 Mar 1;58(3):135-9. doi: 10.2144/000114265. eCollection 2015. 10.2144/000114265 PubMed 25757546 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MSGV Hu Acceptor 6699 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral golden gate cloning vector for expression of functional
Human T-Cell Receptor genes.
ACCESSION .
VERSION .
KEYWORDS MSGV Hu Acceptor
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6699)
AUTHORS Coren LV, Jain S, Trivett MT, Ohlen C, Ott DE
TITLE Production of retroviral constructs for effective transfer and
expression of T-cell receptor genes using Golden Gate cloning.
JOURNAL Biotechniques. 2015 Mar 1;58(3):135-9. doi: 10.2144/000114265.
eCollection 2015.
PUBMED 25757546
REFERENCE 2 (bases 1 to 6699)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6699
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..518
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
primer_bind 1358..1380
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1396..1412
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind 1750..1766
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
misc_feature 2920..2972
/label=MCS
/note="multiple cloning site"
LTR 3016..3530
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 3702..3718
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3726..3756)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3771..3792
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3909..3926)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4080..4668)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4160..4179)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4839..5699)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5462..5481
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5700..5804)
/gene="bla"
/label=AmpR promoter
primer_bind 5872..5890
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5928..5950)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6050..6069
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6263..6285
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6277..6294
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6278..6294
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6493..6512
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
241 ttccagggtg ccccaaggac ctgaaaatga ccctgtgcct tatttgaact aaccaatcag
301 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
361 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca
421 ataaagcctc ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc
481 tcagattgat tgactgccca cctcgggggt ctttcatttg gaggttccac cgagatttgg
541 agacccctgc ctagggacca ccgacccccc cgccgggagg taagctggcc agcggtcgtt
601 tcgtgtctgt ctctgtcttt gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc
661 tgtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg
721 gaacacccgg ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc
781 cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc cttagaggag
841 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
901 ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg
961 tgttgtctct gtctgactgt gtttctgtat ttgtctgaga atatgggccc gggctagcct
1021 gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac
1081 aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca
1141 acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt
1201 aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg
1261 acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag
1321 cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc
1381 ccgcctcgat cctcccttta tccagccctc actccttctc taggcgcccc catatggcca
1441 tatgagatct tatatggggc acccccgccc cttgtaaact tccctgaccc tgacatgaca
1501 agagttacta acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac
1561 gaagtctgga gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct
1621 cacccttacc gagtcggcga cacagtgtgg gtccgccgac accagactaa gaacctagaa
1681 cctcgctgga aaggacctta cacagtcctg ctgaccaccc ccaccgccct caaagtagac
1741 ggcatcgcag cttggataca cgccgcccac gtgaaggctg ccgaccccgg gggtggacca
1801 tcctctagac cctgcaggtt aattaagcca ccaagtcttc tttttttgaa gacttcagaa
1861 ccctgatcct gccgtgtacc agctgcggga cagcaagagc agcgacaaga gcgtgtgcct
1921 gttcaccgac ttcgacagcc agaccaacgt gtcccagagc aaggacagcg acgtgtacat
1981 caccgacaag accgtgctgg acatgcggag catggacttc aagagcaaca gcgccgtggc
2041 ctggtccaac aagagcgatt tcgcctgcgc caacgccttc aacaacagca ttatccccga
2101 ggacacattc ttcccaagcc ccgagagcag ctgcgacgtg aagctggtgg aaaagagctt
2161 cgagacagac accaacctga acttccagaa cctgagcgtg atcggcttcc ggatcctgct
2221 gctgaaggtg gccggcttca acctgctgat gaccctgaga ctgtggtcca gccgggccaa
2281 gagatctggc agcggcgcca ccaatttcag cctgctgaaa caggccggcg acgtggaaga
2341 gaaccctaag tcttcttttt gaagacttga tctgaacaag gtgttccccc cagaggtggc
2401 cgtgttcgag ccttctgagg ccgagatcag ccacacccag aaagccaccc tcgtgtgcct
2461 ggccaccggc tttttccccg accacgtgga actgtcttgg tgggtcaacg gcaaagaggt
2521 gcacagcggc gtgtccaccg atccccagcc tctgaaagaa cagcccgccc tgaacgacag
2581 ccggtactgc ctgagcagca gactgagagt gtccgccacc ttctggcaga acccccggaa
2641 ccacttcaga tgccaggtgc agttctacgg cctgagcgag aacgacgagt ggacccagga
2701 cagagccaag cccgtgaccc agatcgtgtc tgccgaagcc tggggcagag ccgattgcgg
2761 ctttaccagc gtgtcctatc agcagggcgt gctgagcgcc accatcctgt acgagatcct
2821 gctgggcaag gccaccctgt acgccgtgct ggtgtctgcc ctggtgctga tggccatggt
2881 caagcggaag gacttctgaa tttgtagatc gacggatccc tcgagctcaa gcttcgaatt
2941 ctgcagtcga cggtaccgcg ggcccgggat ccgataaaat aaaagatttt atttagtctc
3001 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
3061 ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga tcaaggttag
3121 gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc
3181 cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga
3241 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
3301 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
3361 aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt
3421 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
3481 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat gggtaacagt
3541 ttcttgaagt tggagaacaa cattctgagg gtaggagtcg aatattaagt aatcctgact
3601 caattagcca ctgttttgaa tccacatact ccaatactcc tgaaatccat cgatggagtt
3661 cattatggac agcgcagaaa gagctgggga gaattgtgaa attgttatcc gctcacaatt
3721 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
3781 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
3841 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
3901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
3961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4501 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
4561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
4621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
4681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
4741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
4801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
4861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
4921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
4981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
5161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
5341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
5401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
5461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
5521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
5581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
5641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
5701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
5761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
5821 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
5881 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
5941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
6001 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
6061 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
6121 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
6181 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
6241 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccacg
6301 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
6361 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
6421 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
6481 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
6541 gatgccggcc acgatgcgtc cggcgtagag gcgatttaaa gacaggatat cagtggtcca
6601 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
6661 aaaataaaag attttattta gtctccagaa aaagggggg
//
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